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. 2016 Sep 23;149(4):447–459. doi: 10.1111/imm.12663

Figure 7.

Figure 7

Notch‐1 is crucial for regulatory T (Treg) cell function in experimental autoimmune uveitis (EAU). (a) Knockdown of Notch‐1 or Notch‐2 in Treg cells. Upper panel: representative Immunoblot image. Lower panel: statistical analysis for Notch‐1 and Notch‐2. (b) Experimental design for (c–e). (c) Representative dot plots of donor‐derived Treg cells in uveitic eyes. (d) Expression of Foxp3 and programmed cell death protein 1 (PD‐1) in donor‐derived Treg cells in uveitic eyes. Left panel: representative histograms. Right panel: statistical analysis. (e) mRNA levels of transforming growth factor‐β (TGF‐β), interleukin‐12a (IL‐12a), IL‐27b and interferon‐γ (IFN‐γ) in donor‐derived Treg cells in uveitic eyes. L‐SC, Treg cells transduced with L‐SC. L‐sh‐N‐1, Treg cells transduced with L‐sh‐N‐1. L‐sh‐N‐2, Treg cells transduced with L‐sh‐N‐2. *P < 0·05; **P < 0·01; ***P < 0·001. n = 3 or n = 5 per group. Each circle represents a mouse.