Figure 4.

Validation of quantitative PCR array data by RT‐qPCR array using cDNA prepared from RNA obtained from bone‐marrow‐derived macrophages from wild‐type (WT) and NOD1 knockout (KO) mice in three independent experiments (four to six mice of each genotype were used). These were the same RNA samples combined to prepare the pool of RNA used in the array analysis. Target gene expression was normalized to β‐actin expression and fold regulation was calculated by comparison with the normalized gene expression in WT unstimulated control macrophages.