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. 2016 Oct 3;149(4):423–431. doi: 10.1111/imm.12659

Figure 3.

Figure 3

Gene expression of sterol regulatory element binding protein 1 (SREBP1) and citrate pathway proteins in lymphoblasts from children with Down syndrome (DS). (a) Total RNA extracted from lymphoblastoid cells from six age‐matched control children (black bars) and six children with DS (grey bars), was used to quantify ATP‐citrate lyase (ACLY), citrate carrier (CIC) and SREBP1 mRNAs. (b) ACLY, CIC, SREBP1 and β‐actin from lymphoblastoid cells of six age‐matched controls (C) and six children with DS (DS) were detected by specific antibodies. Western blotting data are representative of four independent experiments. (c) The intensity of immunolabelled protein bands depicted in (b) was quantified by densitometric scanning. Quantification represents the average of fold change for ACLY, CIC and SREBP1 proteins in DS relative to control lympoblastoid cells. In (a) and (c), means ± SD of four replicate independent experiments are shown; where indicated differences between samples and relative controls (**P < 0·01, ***P < 0·001) were significant.