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. 2016 Oct 31;7:12849. doi: 10.1038/ncomms12849

Figure 5. Potential interaction of PARP9 and PARP14.

Figure 5

(a) PARP14 silencing and enforced expression significantly affected PARP9 gene expression in IFNγ-stimulated THP-1 cells (n=3). PARP9 silencing increased PARP14 gene expression (n=3). (b) Co-IP assay revealed a complex between PARP9 and PARP14. (c) Intracellular colocalization of PARP9 and PARP14 in the cytosol in M(-) and M(IFNγ). (d) PARP9 inhibits ADP-ribosylation of STAT1α and STAT6 by PARP14 (protein ribosylation assay). PARP14 auto-ribosylation is also indicated. *P<0.05 and **P<0.01, respectively, by Student's t-test. Error bars indicate s.d.