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. 2016 Oct 31;7:13281. doi: 10.1038/ncomms13281

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Copyright © 2016, The Author(s)

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(a) Force (bar length=120 pN per thick filament), fibre length change (ΔL) and <P₂> for the E-helix probe at different calcium concentrations. (b) Calcium-dependence of ΔL at 0.26 ms (phase 1, filled circles) and 50 ms (phase 2, open circles) (mean±s.d., n=3 fibres). (c) <P₂> for the E-helix probe before the force step (triangles), at the end of phase 1 (filled circles) and phase 2 (empty circles; mean±s.d., n=3 fibres). Initial sarcomere length 2.80 μm, 25 °C, 5% Dextran, 25 μM Blebbistatin.