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. 2016 Oct 31;7:13281. doi: 10.1038/ncomms13281

Figure 4. Thick-filament stress controls the size and speed of the RLC orientation change.

Figure 4

(a) Force (bar length=120 pN per thick filament), fibre length change (ΔL) and <P2> for the E-helix probe at pCa=5.86 for different sizes of force step. (b) Force-dependence of the change in <P2> (Δ<P2>) at the end of phase 1 (filled symbols) and phase 2 (empty symbols; pooled data from five fibres). Solid line: linear regression of phase 2 data with 95% confidence band shown in grey: slope=(−6.6±0.2)·10−4 pN−1, intercept=0.012±0.002. (c) Rate constant of the <P2> change in response to the force increase (symbols as in b). Dashed line: linear regression with 95% confidence band in grey; slope=1.4±0.2 s−1 pN−1, intercept=96±24 s−1. Initial sarcomere length 2.80 μm, 25 °C, 5% Dextran, 25 μM Blebbistatin.