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. 2016 Sep 16;23(10):341–347. doi: 10.1038/cgt.2016.37

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Construction of the heavy (a) and light (b) chain expression plasmids. (a) A sequence was synthesized containing the Kozak-IgE leader sequence, the heavy chain gene encoding the heavy chain variable region (VH) of mumAb4D5 and mCγ1 or mCγ2a. This DNA sequence was subcloned into the EcoRI and NotI sites of pVax1 to construct pVax1-4D5-mIgG1-H or pVax1-4D5-mIgG2a-H, respectively. (b) A DNA sequence was synthesized containing the Kozak-IgE leader sequence, the light chain gene encoding the modified light chain variable region (VL) of mumAb4D5 and mCκ and subcloned into pVax1 to construct pVax1-4D5-mIgG1-L.