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. 2016 Oct 14;23(10):355–364. doi: 10.1038/cgt.2016.41

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Copyright © 2016 The Author(s)

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AdFAST/RFP infection efficiency in common mouse cancer cell lines in vitro. (a) CT26, B16F10 and 4T1 cells were infected with AdRFP or AdFAST/RFP at an MOI of 50 and visualized using fluorescence microscopy 48 hpi. (b) CT26 cells were infected with AdEmpty, AdFAST or VSVΔ51 at varying MOI from 1 to 100 for 72 h. Membrane permeability was assessed by determining the lactate dehydrogenase levels in the cell supernatant. Three independent experiments (n=3) were conducted in triplicate. The values graphed represent the average and the standard error of the mean. Experiments were repeated using the same protocol for (c) B16F10 and (d) 4T1 cells.