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. 2016 Oct 14;23(10):355–364. doi: 10.1038/cgt.2016.41

Figure 3.

Figure 3

Infection of 4T1 cells with AdFAST promotes cell fusion, reduces cellular metabolic activity, and enhances apoptosis. (a) 4T1 cells were infected with AdEmpty or AdFAST at an MOI of 1000, or mock infected with PBS. Cells were fixed and stained with Giemsa stain 48 hpi. Images depicted are representative of two replicates (n=2) (20 × objective). (b) 4T1 cells were infected with AdEmpty, AdmCherry or AdFAST at an MOI of 1000, or mock infected with PBS, and relative metabolic activity was determined using an MTS assay over a time course of 96 h. Results depict the average of three independent experiments (n=3) performed in triplicate. Error bars show the standard error of mean. aP<0.001 compared with 24 h within a treatment group. bP<0.001 for AdFAST compared with AdEmpty- and AdmCherry-treated cells. (c) 4T1 cells were infected at varying MOI with AdFAST-HA, crude protein extracts prepared 72 hpi, and assayed for total caspase-3 and cleaved caspase-3 by immunoblot. Membranes were probed with antibody to tubulin to ensure equal protein loading.