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. 2016 Oct 14;23(10):355–364. doi: 10.1038/cgt.2016.41

Figure 4.

Figure 4

Treatment of 4T1 cells with AdFAST enhances release of exosomes. (a) 4T1 were infected with AdEmpty or AdFAST-HA at an MOI of 1000, or mock infected with PBS and exosomes were isolated from the medium 72 hpi using Exoquick. An aliquot of the resulting purified exosomes were analyzed by Zetaview (Particlemetrix, Germany) for particle size. Shown is one representative histogram of size distribution for each treatment along with the average +/− standard deviation of the particle size for 3–4 independent experiments. (b) 4T1 were infected with AdEmpty or AdFAST-HA at an MOI of 1000, or mock infected with PBS, and exosomes were isolated from the medium 72 hpi. Equal volume of the resulting exosome samples were separated by SDS–PAGE, and analysed by immunoblot for exosome markers Alix and Flotillin, and for the HA tag on FAST protein. (c) The concentration of exosome particles isolated from media was quantified using Zetaview according to the manufacturer's instructions. Exosomes were isolated from 3–4 independent experiments. Bars represent mean±standard deviation. ** indicates significant differences between groups (P<0.0001).