Figure 5.
AdFAST does not promote survival or reduce tumor growth in immunocompetent Balb/C mice with subcutaneous 4T1 tumors. (a) Kaplan–Meier survival curve for 4T1 tumor-bearing mice treated with AdFAST. Balb/C mice with subcutaneous 4T1 tumors were intratumorally injected with PBS or 1.9 × 109 pfu AdFAST. Each treatment group consisted of five mice. (b) The average size of tumors treated with PBS or AdFAST as described in (a). The error bars represent the standard deviation. The + symbols show the number of mice that had reached endpoint by the time measurements were taken. (c) Subcutaneous 4T1 tumors from Balb/C mice were removed, fixed, sectioned and stained with hematoxylin and eosin stain 3 days post injection. Images are representative from a group of three mice per treatment. (d) Balb/C mice with subcutaneous 4T1 tumors were intratumorally injected with 1.4 × 108 pfu AdEmpty. Three days post injection, tumors were excised, fixed, sectioned and subjected to hematoxylin and eosin stain.