Table 1. Ingredient of media used for isolation and fermentation of microbial strains from E. superbaa.
| Microbes | Medium | Ingredient (L−1) |
|---|---|---|
| Actinobacteria | GYM | 4.0 g dextrose,4.0 g yeast extract,10.0 g malt extract,2.0 g CaCO3,12.0 g agar |
| ISP2 | 4.0 g yeast extract,10.0 g malt extract,4.0 g dextrose,20.0 g agar | |
| Chitin | 2.0 g chitin, 1.0 g NH4Cl, 0.9 g K2HPO4·3H2O, 0. g KH2PO4, 0.5 g MgSO4·7H2O 0.001 g FeSO4, 0.001 g ZnSO4, 0.1 g CaF2, 20.0 g agar | |
| M5 | 20.0 g agar | |
| SC | 10.0 g soluble starch, 0.3 g casein, 2.0 g KNO3, 2.0 g NaCl, 2.0 g K2HPO4, 0.05 g MgSO4·7H2O, CaCO3 0.02 g, 0.01 g FeSO4·7H2O, 20.0 g agar | |
| Actinobacterial medium 2#b | 20.0 g glucose, 10.0 g soluble starch, 10.0 g peptone, 10 g yeast extract, 3.0 g beef extract, 0.5 g KH2PO4, 0.5 g MgSO4, 2.0 g CaCO3, pH 7.5–8.0. | |
| Fungi | PDA | 200 g potato extract, 20 g dextrose, 20.0 g agar |
| Emerson | 1.0 g dextrose,1.0 g yeast extract powder,4.0 g peptone,2.5 g NaCl,18.0 g agar | |
| Sabouraud | 10.0 g peptone, 40.0 g maltose, 20.0 g agar | |
| Fungal medium 2#b | 20.0 g maltose,10.0 g aginomoto,0.5 g KH2PO4, 0.3 g MgSO4·7H2O,10.0 g dextrose, 3.0 gyeast extract, 1.0 g corn steep liquor,20.0 g mannitol, pH6.5 | |
| SWSb | 1.0 g peptone, 10.0 g soluble starch, pH 7.5–8.0 | |
| Bacteria | Marine agar 2216c | 5.0 g peptone, 1.0 g yeast extract, 0.1 g ferric citrate, 0.08 g KBr, 19.45 g NaCl, 8.8 g MgCl2, 3.24 g Na2SO4, 1.8 g CaCl2, 0.55 g KCl, 0.16 g NaHCO3, 0.0016 g, NH4NO3, 0.008 g Na2HPO4, 0.022 g H3BO3, 20.0 g agar |
| Luria Bertani (LB)b,c | 5.0 g yeast extract, 10.0 g peptone, 5.0 g NaCl, 15.0 g agar | |
| Zobell 2216E | 5.0 g peptone,3.0 g yeast extract,15.0–20.0 g agar | |
| Glucose Yeast Extract Starch | 10.0 g dextrose, 10.0 g yeast extract,10.0 g starch, 5.0 g NaCl, 3.0 g CaCO3, 20.0 gagar, pH 7.0 | |
| Penssay Broth | 1.5 g beef extract, 1.5 g yeast extract, 5.0 g tryptone, 3.5 g NaCl, 1.0 g dextrose, 4.8 g K2HPO4·3H2O, pH 7.2 |
aAll media were prepared with natural sterile seawater and adjusted corresponding pH prior to autoclaving at 121 °C for 20 min.
bThe liquid media were used to ferment actinobacteria, bacteria and fungi in bioassay and compound isolation, respectively.
cThe 2216E and LB media were also used to culture aquatic and human pathogenic bacteria in the antibacterial tests, respectively.