Figure 5. Fertilising ability of Slc22a14-deficient sperm.

Wild-type or Slc22a14^−/− male mice were mated with wild-type female mice, and the number of spermatozoa in the uterus horns 2.5 h after of coitus (a) or the number of two-cell embryos recovered from oviducts 1.5 days after coitus (b) was counted. Data indicate the average of three experiments. Error bars represent standard errors. (c) Fertilisation rate in vitro using wild-type or Slc22a14^−/− spermatozoa and wild-type oocytes. The number of two-cell embryos were counted 24 h after insemination. Data are presented as average ± SE (n = 3). (d) Capacitation status of sperm. Sperm were incubated in TYH medium for the indicated times, and protein tyrosine phosphorylation (p-Tyr) levels were monitored using western-blotting.