Table 1. Metal-conjugated antibodies and element-containing reagents used to stain tissue sections for imaging mass cytometry.

Antibody/Reagent	Antibody Clone	Metal	Catalog number	Final concentration (μg/mL)
γH2AX	JBW301	147Sm	3147016A	5
p53	DO-7	150Nd	3150024A	5
Vimentin	RV202	156Gd	3156023A	5
*E-cadherin	24E10	158Gd	3158021A	5
EF5	ELK3-51	159Tb		10
VEGF	23410	161Dy	3161002B	5
*Pan-Keratin	C11	162Dy	3162027A	5
CK7	RCK105	164Dy	3164020A	5
β-catenin	D13A1	165Ho	3165027A	5
Ki-67	B56	168Er	3168007B	5
#Collagen I	Goat poly	169Tm		5
BRCA1	MS110	172Yb		5
#αSMA	1A4	175Lu		5
Histone H3	D1H2	176Yb	3176016A	5
*Intercalator		191Ir, 193Ir	201192B	25 µM
IdU		127I		60 mg/kg

^*Denotes parameters used in cell segmentation by the Definiens Developer software.

^#Denotes parameters used to identify collagen and non-collagen regions.