Table 3. The IC50 values of CLA-PTX@PEG NPs in in MDA-MB-231, B16-F10 and U87-MG cell lines. (n = 3).
| Formulations | IC50 (μM) | ||
|---|---|---|---|
| MDA-MB-231 cells | B16-F10 cells | U87-MG cells | |
| Taxol | 1.51 ± 0.20 | 1.36 ± 0.17 | 2.85 ± 0.60 |
| Free CLA-PTX | 4.06 ± 0.21$$ | 4.14 ± 0.17$$ | 6.83 ± 0.16$$ |
| CLA-PTX@PEG NPs | 2.41 ± 0.52**$$ | 3.66 ± 0.40*$$ | 4.62 ± 0.59**$$ |
*p < 0.05 or **p < 0.01, vs Free CLA-PTX group; $$p < 0.01, vs Taxol group.
Taxol: Taxol (6 mg/ml) was diluted with 5% glucose injection to 320 μM of PTX. This PTX solution was then diluted with culture medium to a series concentrations of PTX.
Free CLA-PTX: 40 mg CLA-PTX was dissolved in 5 ml of CrEL/dehydrated ethanol (50:50, v/v), and then diluted with 5% glucose injection to 320 μM of CLA-PTX. Following that, this CLA-PTX solution was diluted with culture medium to a series concentrations of CLA-PTX.
CLA-PTX@PEG NPs: CLA-PTX@PEG NPs (6 mg/ml) was firstly diluted with 5% glucose injection to 320 μM of CLA-PTX. This CLA-PTX@PEG NPs was then diluted with culture medium to a series concentrations of CLA-PTX.