Figure 4. Demonstration that formamide can significantly accelerate a 37 °C regular CHA reaction inhibited by small-scale self-folding structures.

(A) Sequences and structures of three catalysts, C_linear, C_structured1, and C_structured2, respectively. The three catalysts hold the same C_linear sequence that can trigger the CHA reaction at 37 °C. The segment in red was the toehold binding domain, which was partly blocked in C_structured1 and C_structured2 at different degrees. (B) Real-time fluorescent kinetic monitoring right after buffer, C_linear, C_structured1, or C_structured2 was added into CHA components without (B) and with (C) 20% formamide.