Figure 6. Demonstration that LAMP-OHT-CHA combination is environmental ultra-compatible.
Fluorescent responses of OHT-CHA1 to LAMP products amplified from synthetic MERS-1A DNA negative (0 copy) and positive (2000 copies, 6 × 10−17 M) samples. Data were collected, respectively, on (A) a professional real-time PCR system at 55 °C simultaneously with LAMP reaction, (B) a fluorescence (FL) spectrum under room temperature fluctuating from 19 °C to 21 °C, and (C) a portable FL detector under outdoor temperature fluctuating from 6.5 °C to 12 °C. For Fig. B and C, Data were at end-point collected after LAMP products were manually added into CHA reaction. Other useful notes about OHT-CHA-LAMP detection see the last page of supporting information).