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. 2016 Oct 11;5(10):e373. doi: 10.1038/mtna.2016.64

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Functional analysis of miR-140 2 days after electroporation. (a) RT-qPCR analysis of miR-140 levels in ACs electroporated with a negative control sequence (Control), miR-140 and in native articular cartilage. (b) Relative expression of RALA, BMP2, and ADAMTS5 in control cells and miR-140 electroporated cells. Cells from three donors were used in a and b. U6 and GAPDH were used as a reference genes in a and b, respectively. In a and b the cells were stimulated for 20 hours before analysis. In c the cells were stimulated for 0, 30 minutes, 1, 3, 5, 8, 20, and 48 hours. In d the cells were stimulated for 1, 2, 5, and 7 days. Min, minutes; h, hours. RT-qPCR, real time-quantitative polymerase chain reaction; ACs, articular chondrocytes.