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. 2016 Oct 11;5(10):e373. doi: 10.1038/mtna.2016.64

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Western blot analysis of inflammation and chondrogenesis proteins. (a) IKB protein levels were analyzed in unstimulated cells, after 30 minutes (min) and 20 hours (h) following rhIL1B stimulation. (b) IL1B, IL6, IL8, ACAN, SOX9, and CSGALNACT1 in cells electroporated with control or miR-140 for 1 day and subsequently stimulated with rhIL1B for 20 hours. ACTB was used as loading control in a and b. (c) RT-qPCR analysis of ACAN, SOX9, and CSGALNACT mRNA levels after electroporation with miR-140 and stimulation with rhIL1B. D1, Donor 1; D2, Donor 2; D3, Donor 3. IL1B, interleukin 1β; RT-qPCR, real time-quantitative polymerase chain reaction; rhIL1B, recombinant human IL1B.