Skip to main content
. 2016 Oct 11;5(10):e375. doi: 10.1038/mtna.2016.82

Figure 5.

Figure 5

MDR-1 is a direct target of miR-451 and influences chemoresistance. (a) The 3′-UTR of MDR-1 is discovered complementary binding site for miR-451. (b) The relative luciferase activity in pLUC-MDR1-3′UTR-wt or pLUC-MDR1-3′UTR-mut transfected SPC-A1/DTX cells with transfection of miR-451 overexpression plasmid (pcDNA/miR-451). (c) The mRNA and protein level of MDR-1 in SPC-A1 cells and SPC-A1/DTX. (d) The protein level of MDR-1 in SPC-A1 cells with transfection of Notch-1 overexpression plasmid (pcDNA3/Notch-1) or treatment of miR-451 inhibitor and SPC-A1/DTX cells with transfection of miR-451 overexpression plasmid (pcDNA/miR-451) or transfection of Notch-1 short hair RNA plasmid (pGPU6/sh-Notch-1). (e) The protein level of MDR-1 in SPC-A1/DTX cells treated with DAPT in different concentrations (0, 2, 5 or 10 μmol/l). (f) The protein level of MDR-1 in SPC-A1/DTX cells treated with 10 μmol/l DAPT or 10 μmol/l DAPT plus miR-451 inhibitor. *P < 0.05 and **P < 0.01. DAPT, N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; MDR, multidrug resistant protein-1; mRNA, microRNA.