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. 2016 Nov 4;6:36002. doi: 10.1038/srep36002

Figure 3. IL-25 inhibits Th17 cells without affecting Th1 cells in RA.

Figure 3

CD4+ T cells from PBMCs of RA patients or HCs were isolated and stimulated with or without anti-CD3 (5 μg/mL) plus anti-CD28 (2 μg/mL) in the presence or absence of rhIL-25 (100 ng/mL) for 24 h. IL-17A (A), IFN-γ (B), and IL-4 (C) levels in supernatant were assessed by ELISA. The mRNA levels of IL-17A (D), IFN-γ (E), and IL-4 (F) were detected by real-time PCR. The mRNA levels of the master transcription factors ROR-γt (G), T-bet (H), and GATA3 (I) were detected by real-time PCR. Data are presented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.