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Knock out (KO) of OsGRF4 in NIL‐527R decreases grain size and weight. (a) Sequence comparisons of the targeting site of the KO plants; the sequence in red is the gRNA target site. Note that KO1‐1 plant is bi‐allelic and others are homozygous. (b) Grain comparisons of the control and OsGRF4 KO plants; scale bar, 10 mm; (c) KGW of plants in (b); (d) grain length of plants in (b); (e) grain width of plants in (b); values are all shown as means ± SEM (**P < 0.01).
