Figure 4. Glycerolipid molecular species in soybean root hairs and stripped roots with and without N supply.

Four-day-old seedlings grown on nitrogen-free B&D agar medium were treated with 10 mM NH₄NO₃ (20 mM N) or water (No N) for 12 hours. Glycerolipid amounts are expressed as normalized mass spectral signal/total normalized glycerolipid mass spectral signal (to produce percentage of normalized MS signal, mol% of total lipids). The values are the mean ± SD (n = 10). The data of soybean stripped roots and root hairs were compared via t test and the P < 0.05 is indicated by *, indicating a significant difference. The value for nitrogen-sufficient seedlings is higher (represented as H) or lower (represented as L) than the value for nitrogen-deficient seedlings.