Figure 2. ERK1/2 activation is the major output of TPL-2 signalling following LPS stimulation of macrophages.

(A) Heatmap showing log₂ ratios averaged from both WT/Map3k8^D270A/D270A and WT ± PD0325901 comparisons (Figure 1) for all phosphopeptides ≥4× down-regulated by Map3k8^D270A mutation. Data underwent hierarchical clustering by rows and column. (B) TPL-2 phosphorylation motif deduced from analysis of all phosphopeptides ≥4× down-regulated by Map3k8^D270A mutation. Canonical ERK1 and RSK phosphorylation motifs from published studies are also shown for comparison.