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. 2016 Sep 27;473(19):3159–3175. doi: 10.1042/BCJ20160648

Figure 3. PKA binding of nucleotides is detectable by DSF.

Figure 3.

(A) TSA of PKA WT, K72H and R133A (5 μM) in the presence of 10 mM MgCl2 and 1 mM (blue), 2 mM (green) or 4 mM (orange) ATP; buffer control is in red. (B) ΔTm for WT, K72H and R133A PKA upon nucleotide binding, as measured by DSF. Mean ΔTm values ± SD (n = 2) were calculated by subtracting the control Tm value (buffer, no nucleotide) from the measured Tm value.