Table 1. Sites of autophosphorylation on PKAc WT before and after treatment with λPP, and of PKAc R133A, identified by LC–MS/MS analysis of tryptic peptides.
| Sample | Sequence | Site |
|---|---|---|
| WT, R133A | *KGpSEQESVK | *Ser10 |
| R133A | WEpTPSQNTAQLDQFDR | †Thr32 |
| WT, R133A | WETPpSQNTAQLDQFDR | Ser34 |
| WT, R133A | WETPSQNpTAQLDQFDR | †Thr37 |
| WT | IKpTLGTGSFGR | Thr48 |
| WT, R133A | IKpTLGTGpSFGR | Thr48, Ser53 |
| WT, R133A | IKTLGpTGSFGR | Thr51 |
| WT, R133A | *TLGTGpSFGR | *Ser53 |
| WT, R133A | HKEpSGNHYAMK | †Ser65 |
| WT, R133A | *IGRFpSEPHAR | *Ser139 |
| WT, R133A | *TWpTLCGTPEYLAPEIILSK | *Thr197 |
| WT | *FPpSHFSSDLK | *Ser259 |
| WT, R133A | *VpSINEK | *Ser338 |
*
Sites preserved following the treatment of WT PKAc with Mn2+–λPP.
†
Indicates novel phosphosites.