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. 2016 Oct 27;473(21):3997–4009. doi: 10.1042/BCJ20160696

Figure 6. Peroxidase-derived superoxide stimulates HemY-mediated oxidation of protoporphyrinogen IX.

Figure 6.

Kinetic assays showing the effect of HRP (1 µM), superoxide dismutase (1 µM), and catalase (1 µM) upon the activity of HemY (0.5 µM) using protoporphyrinogen IX (8.6 µM) and coproporphyrinogen III (8.2 µM) substrates. Abbreviations: HRP, horseradish peroxidase; SOD, superoxide dismutase; Cat, catalase; P'gen IX, protoporphyrinogen IX; C'gen III, coproporphyrinogen III. Asterisks indicate that rates measured are significantly different from those measured in the absence of peroxide (Student's t-test, ***P < 0.001).