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. 2016 Oct 27;473(21):3965–3978. doi: 10.1042/BCJ20160657

Figure 6. LITAF is not a TA protein in cells.

Figure 6.

(A) Lysates from HeLaM cells transfected with Sec61β-OPG2 (left panel) or with LITAF1–135-Sec61βL-OPG2 (right panel) were immunoprecipitated with anti-OPG2. Samples were treated with or without PNGaseF and then immunoblotted with anti-OPG2 (left panel) or anti-Strep-tag (right panel). Arrowheads indicate non-glycosylated products. Arrows indicate glycosylated Sec61β-OPG2. (B) Left panel: Lysates from HeLaM cells transfected with Sec61β-OPG2 or strep-LITAF1–135-βlumen-OPG2 were treated with or without EndoH and immunoblotted with anti-OPG2. Closed arrowhead denotes Sec61β-OPG2 and arrow denotes glycosylation product. Open arrow denotes LITAF1–135-βlumen-OPG2. Right panel: to further confirm the identity of LITAF-specific products, extracts from untransfected cells or cells transfected with strep-LITAF1–135-βlumen-OPG2 were treated with or without EndoH and immunoblotted with anti-Strep-tag. Open arrow denotes LITAF chimaera.