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. Author manuscript; available in PMC: 2016 Nov 4.
Published in final edited form as: Science. 2014 Dec 5;346(6214):1258236. doi: 10.1126/science.1258236

Fig. 2. Dredd-mediated Rel activation eliminates loser cells.

Fig. 2

(A) Rel and Tollo are required for death of loser cells. TUNEL assays showing cell death in control clones, WT loser clones, and loser clones in RelE20 and tolloR23B mutant backgrounds 24 hours after clone induction. Many WT loser cells are TUNEL-positive, but their death is suppressed in the mutant backgrounds. (B) Quantification of data from (A). (C) Expression of Rel-RNAi (or Rel-RNAi and Dicer to enhance RNAi efficiency) within loser cells suppresses their loss but does not affect control clones. (D) Dredd and Rel are critical for elimination of WT loser cells. Loss of Dredd suppresses the loss of loser clones but does not affect the growth of control clones. Whereas DreddB118−/+ has little effect on loser death, in combination with RelE20−/+ it strongly suppresses death. (E) Expression of Dredd in the absence of competition reduces clone size, but this is blocked by coexpression of Rel RNAi. The number of clones scored for each genotype is indicated. All P values are relative to WT loser clone size except in (C): ***P < 0.001. ns, not significant (Mann-Whitney test).