Table 1.

Efficacy of CRISPR/Cas9 system to induce targeted disruption of IL2RG during embryogenesis in vitro. CRISPR/Cas9 system at the concentration of 10 ng/ul each sgRNAs and 20 ng/ul Cas9 mRNA was introduced into pig zygotes. Genotyping of subsequent embryos on day 7 demonstrated that all embryos carried mutation on IL2RG; no wild type sequence was found from genotyping

Concentration of CRISPR/Cas9 (ng/uL)	# of embryos injected	% of blastocyst on day 7 (number of blastocysts/cleaved)	# of blastocyst genotyped	Genotypes
				Homozygous Mutation	Biallelic Mutation	Mosaic Mutation	Heterozygous Mutation	Wild-type
10/20	70	25.71 % (18/70)	4	0	2	2	0	0