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. 2016 May 3;18(11):1570–1582. doi: 10.1111/cmi.12597

Figure 3.

Figure 3

Role of CEACAM3 ITAM in Moraxella catarrhalis UspA1‐induced CXCL8 release in NB4 cells. A. Flow cytometry of CEACAM3 and CD11b expression on the surface of untreated and all‐trans retinoic acid (ATRA)‐differentiated NB4 cells. Mouse IgG1 antibody was used as an isotype control. B. Flow cytometry data of CEACAM3 knock‐down efficiency (grey line: isotype control, thick line: control‐siRNA‐treated cells or CEACAM3‐siRNA treated cells) and ELISA of CXCL8 in supernatants of ATRA‐differentiated NB4 cells transfected for 96 h with control or CEACAM siRNA, and subsequently infected with M. catarrhalis strain BBH18 (MOI 50) for 16 h. C. ELISA of CXCL8 in supernatants of NB4 cells transiently transfected with control vector or wild‐type CEACAM3 left untreated or incubated for 16 h with the M. catarrhalis wild‐type strain BBH18 or ∆UspA1 mutant strain BBH18.1 (MOI 50). D. ELISA of CXCL8 in supernatants of NB4 cells. Cells were transiently transfected with CEACAM3 wt, CEACAM3 Y230F, CEACAM3 Y241F or control vector, then left untreated or stimulated for 16 h with M. catarrhalis strain BBH18 (MOI 50). Uninfected cells transfected with control vector have been set to 1 (range 246‐1323 pg/ml). Data are representative from one of three independent experiments (A) or are from at least three experiments (mean ± SD) performed in duplicates (B, C) (p < 0.05).