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. 2016 May 3;18(11):1570–1582. doi: 10.1111/cmi.12597

Figure 5.

Figure 5

CARD9 is required for the M. catarrhalis‐induced CXCL8 release in NB4 cells. A. Gene‐silencing abilities of the CARD9 and control RNA duplexes in ATRA‐differentiated NB4 cells were assessed by qPCR analysis 72 h post‐transfection, shown as relative quantification (RQ). B. ATRA‐differentiated NB4 cells were transfected with the siRNAs as indicated for 72 h and subsequently infected with M. catarrhalis strain BBH18 (MOI 50) for 16 h. CXCL8 concentrations in the supernatants were analysed by ELISA. Data represent mean ± SD of at least three individual experiments performed in duplicates * p < 0.05 C. Luciferase activity in HEK293 cells transiently co‐transfected with CARD9/BCL10 or CEACAM3 wild‐type, CEACAM3 Y230F, CEACAM3 Y241F or control vector along with an NF‐κB‐driven luciferase reporter plasmid and a Renilla luciferase reporter plasmid, then left untreated or stimulated for 5 h with M. catarrhalis strain BBH18 (MOI 50). Relative luciferase activities (relative luciferase units, RLU) were obtained and are presented as percent of control (uninfected cells transfected with CARD9, BCL10 and CEACAM3 were set to 100%). Columns represent mean ± SD of three independent experiments done in triplicates (p < 0.05).