Figure 1.

Effects of the methanolic extract of Betula platyphylla on adipocyte differentiation in 3T3-L1 cells3T3-L1 pre-adipocytes were treated with various concentrations of the metabolic extract of B. platyphylla during differentiation (day 0–8). On day 8, cultures were stained with Oil Red O and the lipid contents were quantified spectrophotometrically at 544 nm. Relative lipid contents (%) were calculated as 100 × [(absorbance of sample-treated – absorbance of undifferentiated control)/(absorbance of differentiated control – absorbance of undifferentiated control)]. Results represent the mean ± SD of three independent experiments, each performed in triplicate wells. *p < 0.05, **p < 0.01, ***p < 0.001, versus differentiated cells.