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. Author manuscript; available in PMC: 2016 Nov 4.
Published in final edited form as: Stem Cells. 2016 May 19;34(7):1846–1858. doi: 10.1002/stem.2391

Figure 1.

Figure 1

MiR-211 modulated mesenchymal stem cell (MSC) migration. Migrated MSCs assessed by transwell assay (A, scale bar=100 μm), and cell number was quantified for each group (B, n>3 per group). Fluorescent image showed green fluorescent protein (GFP) positive MSCs in the peri-infarct area (C, scale bar=50 μm), and number of GFP positive cells was quantified (D, n≥3 heart samples per group). TnT and Hoechst costaining were performed as shown in red and purple, respectively. SRY gene copies were quantified by real time polymerase chain reaction, the migrated MSCs cells were determined as the absolute number of SRY gene copies per mg myocardium tissue (E, n=4 per group). MSCsmiR-211-over was abbreviated as over, MSCsmiR-211-shRNA as shRNA, and their controls, MSCsmiR-211-control and MSCsmiR-211-scramble, are abbreviated as CV and NC, respectively. Abbreviations: GFP, green fluorescent protein; TnT, Troponin T.