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. Author manuscript; available in PMC: 2016 Nov 4.
Published in final edited form as: Stem Cells. 2016 May 19;34(7):1846–1858. doi: 10.1002/stem.2391

Figure 2.

Figure 2

MiR-211 overexpressing mesenchymal stem cells (MSCs) improve post-myocardial infarction (MI) remodeling. Echocardiography examination was performed for each group rats at 28 days after MI (A), and fractional shortening was quantified (B). Left ventricle (LV) pressure recording was also obtained, and LV-end-diastolic pressure (C), and ±dP/dtmax (D, E) then measured. Masson trichrome staining was performed (F) to evaluate the infarct size (G). Immunofluorescence microscopy with antibody targeting CD31 was performed, and CD31 positive structure was quantified in the peri-infarct area using the heart tissue obtained at day 28 after infarction, where troponin I and Hoechst were costained for identifying myocardium and nuclei (H, I, scale bar=75 μm). Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) staining was also done and positive cells quantified (K) in the peri-infarct area using the heart tissue obtained at day 3 after infarction (J, scale bar=100 μm). All the abbreviations are the same as in Figure 1. Abbreviations: HRF, high resolution field; LVFS, left ventricular fractional shortening; PBS, phosphate buffer solution; shRNA, short hairpin ribonucleic acid; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.