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. Author manuscript; available in PMC: 2016 Nov 4.
Published in final edited form as: Stem Cells. 2016 May 19;34(7):1846–1858. doi: 10.1002/stem.2391

Figure 3.

Figure 3

Identification of STAT5A as a target of miR-211. Schematic of the miR-211 putative binding site alignment with rat 3′-UTR mRNA of STAT5A, the mutated STAT5A 3′-UTR binding site for miR-211 are illustrated with the mutated nucleotides underlined (A). Using Hela cells, dual luciferase report assay was performed. Renilla and Firefly Luciferase activities were measured 48 hours after transfection with Renilla used as baseline control (B, n=3). Using real time polymerase chain reaction, the effect of miR-211 on mRNA level of STAT5A was tested in mesenchymal stem cells after infected with miR-211-shRNA or miR-211-over (in grey bars and their respective controls shown in black bars, respectively. C). Western blot was also done (representative bands in D) to quantify the effect of miR-211 on STAT5A protein expression in MSCs (E, the layout is the same as in (C), n=3). All the abbreviations are the same as above. Abbreviations: shRNA, short hairpin ribonucleic acid; UTR, untranslated region; WT, wild type.