Figure 7.

Low miR-211 expression impairs migration of aged hMSCs ability. Downregulated miR-211 expression levels were detected by quantitative real time polymerase chain reaction in aged hMSCs (hMSCs^old, n=3) compared with young hMSCs (hMSCs^young, n=3. A). Migration ability of hMSCs was assessed by transwell assay when both young and old hMSCs were infected with miR-211-over (denoted as hMSCs^{young+miR-211} and hMSCs^old+miR-211, respectively) compared with hMSCs^young and hMSCs^old controls, respectively, with representative images shown in (B) (scale bar=200 μm) and the fold changes in number of migrated cells (mean value from three wells and total number of five fields counted for each well, C). Scale bar=200 μm. Abbreviation: hMSCs, human mesenchymal stem cells.