Figure 6A: Western blot analysis of SIRT1 expression in whole liver homogenates from 6 month old offspring that were sham exposed or exposed to cigarette smoke from GD1 through PD21. Liver homogenates from CSE offspring at age 6 months exhibited a decrease of approximately 10% in expression of the metabolic regulatory protein SIRT1 (*p<0.05; n=3 per group).
Figure 6B: Western blot analysis of PEPCK expression in whole liver homogenates from 6 month old offspring that were sham exposed or exposed to cigarette smoke from GD1 through PD21. Liver homogenates from CSE offspring at age 6 months exhibited an increase of approximately 44% in PEPCK expression in the fed state (*p<0.05; n=4 per group).
Figure 6C: Western blot analysis of PGC1α expression in whole liver homogenates from 6 month old offspring exposed to cigarette smoke from GD1 through PD21. Liver homogenates from CSE offspring at age 6 months exhibited an increase of approximately 86% in PGC1α expression in the fed state (*p<0.05; n=4 per group).