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. 2016 Nov 4;7:1663. doi: 10.3389/fpls.2016.01663

FIGURE 3.

FIGURE 3

Contents of chlorophyll a(A), b(B), a+b(C) and carotenoids (D) in leaf disks cut from 24-day-old pea plants grown from the control (C), hydroprimed (H), and hydroprimed with an aqueous solution of 50 μM melatonin (H-MEL50) or 200 μM melatonin (H-MEL200) seeds. Measurements were performed at T0 (before PQ treatment) and after 24 and 48 h of disk incubation in 75 μM PQ solution. The results are expressed as mean values of about 5 measurements ± SEM. Two-way ANOVA and Duncan’s post hoc test were performed. The small letters on the graphs show statistical significance. ANOVA results: Chlorophyll a – Variant (C, H, H-MEL50, and H-MEL200) F(3,24) = 11.6 p < 0.0001; Time (T0, 2, 4, and 6 h) F(2,24) = 127 p < 0.0001; and interaction Variant × Time F(6,24) = 2.68 p < 0.05; Chlorophyll b – Variant (C, H, H-MEL50, and H-MEL200) F(3,24) = 12.8 p < 0.0001; Time (T0, 2, 4, and 6 h) F(2,24) = 51 p < 0.0001; and interaction Variant × Time F(6,24) = 3.91 p < 0.01; Chlorophyll a+b – Variant (C, H, H-MEL50, and H-MEL200) F(3,24) = 12.6 p < 0.0001; Time (T0, 2, 4, and 6 h) F(2,24) = 97.6 p < 0.0001; and interaction Variant × Time F(6,24) = 2.84 p < 0.05; Carotenoids – Variant (C, H, H-MEL50, and H-MEL200) F(3,24) = 4.31 p < 0.01; Time (T0, 2, 4, and 6 h) F(2,24) = 218 p < 0.0001; and interaction Variant × Time F(6,24) = 1.78 p = 0.14.