Table 2. 28 sperm microRNAs from fathers predicted to be differentially abundant in HFD F0 sperm (n = 4) compared with CD sperm (n = 4).
| Array Card Assay miR ID | CD (Ct) | HFD (Ct) | HFD FC | p value |
|---|---|---|---|---|
| Up-regulated in HFD | ||||
| *mmu-miR-126-3p-4395339 | 21.87 ± 0.26 | 20.05 ± 0.32 | 3.53 | 0.004 |
| *mmu-miR-135b-4395372 | 27.45 ± 0.31 | 25.77 ± 0.33 | 3.18 | 0.010 |
| *mmu-miR-143-4395360 | 22.43 ± 0.26 | 20.29 ± 0.73 | 4.40 | 0.018 |
| *mmu-miR-133b-4395358 | 27.72 ± 0.75 | 25.74 ± 0.29 | 3.97 | 0.029 |
| *mmu-miR-136-4395641 | 28.38 ± 0.26 | 27.16 ± 0.31 | 2.33 | 0.030 |
| *mmu-miR-126-5p-4373269 | 24.76 ± 0.49 | 23.32 ± 0.29 | 2.72 | 0.036 |
| mmu-miR-376b-4395582 | 35.90 ± 1.65 | 32.42 ± 0.44 | 11.12 | 0.047 |
| *mmu-miR-141#-002513 | 29.34 ± 0.29 | 28.35 ± 0.18 | 1.99 | 0.047 |
| *mmu-miR-145-4395389 | 19.47 ± 0.49 | 17.82 ± 0.58 | 3.14 | 0.050 |
| *mmu-miR-337-000193 | 31.98 ± 0.69 | 30.32 ± 0.36 | 3.18 | 0.050 |
| *mmu-miR-30a-4373061 | 20.99 ± 0.46 | 19.81 ± 0.60 | 2.25 | 0.059 |
| *mmu-miR-376a-4373347 | 31.12 ± 0.56 | 29.13 ± 0.60 | 4.14 | 0.059 |
| Down-regulated in HFD | ||||
| mmu-miR-669n-197143_mat | 27.34 ± 0.43 | 29.54 ± 0.37 | -4.60 | 0.005 |
| mmu-miR-669C-002646 | 28.87 ± 0.47 | 30.79 ± 0.28 | -3.79 | 0.008 |
| mmu-miR-669l-121149_mat | 29.96 ± 0.51 | 32.14 ± 0.44 | -4.53 | 0.010 |
| mmu-miR-150-4373127 | 16.81 ± 0.33 | 18.27 ± 0.21 | -2.77 | 0.011 |
| mmu-miR-467H-002809 | 31.25 ± 0.65 | 34.65 ± 1.07 | -10.56 | 0.015 |
| *mmu-miR-1961-197391_mat | 32.17 ± 0.51 | 34.97 ± 0.93 | -7.00 | 0.018 |
| *mmu-miR-184-4373113 | 19.78 ± 0.26 | 21.00 ± 0.32 | -2.32 | 0.031 |
| mmu-miR-466a-3p-002586 | 27.05 ± 0.44 | 28.37 ± 0.21 | -2.49 | 0.033 |
| mmu-miR-672-4395438 | 22.93 ± 0.22 | 23.87 ± 0.13 | -1.93 | 0.036 |
| mmu-miR-412-002575 | 32.94 ± 0.31 | 34.30 ± 1.14 | -2.57 | 0.039 |
| mmu-miR-669D-002808 | 28.82 ± 0.61 | 30.60 ± 0.49 | -3.42 | 0.040 |
| mmu-miR-139-3p-4395676 | 31.59 ± 0.99 | 35.01 ± 1.34 | -10.77 | 0.045 |
| mmu-miR-1969-121131_mat | 29.40 ± 0.21 | 30.66 ± 0.20 | -2.38 | 0.051 |
| mmu-miR-879#-002473 | 32.43 ± 0.58 | 35.61 ± 0.54 | -9.04 | 0.053 |
| mmu-miR-92a-4373013 | 18.77 ± 0.37 | 19.53 ± 0.47 | -1.69 | 0.054 |
| mmu-miR-466b-3-3p-002500 | 26.72 ± 0.34 | 27.90 ± 0.21 | -2.26 | 0.059 |
p value (not FDR adjusted) ranked normalised Ct values are given for the 12 up-regulated and 16 down-regulated murine microRNAs as indicated by Taqman miRNA PCR array cards. All data is presented as Mean ± SEM. Mean dCt were derived from the geometric mean of mmu-miR-10a-5p and mmu-miR-195-5p (the least variable microRNAs across all samples). A full list of microRNA detectors can be found in S1 Table.
CD Control Diet, HFD High Fat Diet, FC fold change (of HFD vs CD by ΔΔCt method), p value from LIMMA analysis of normalised Ct values.
* Confirmed as differentially abundant by subsequent qPCR interrogation in an extended cohort of F0 mice.