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A. Sequence variant strains expressing Hsp104WT (ΔRPR (SY2023), R1-5 (SY2022), RWT (SLL2606), R2E1 (SY2247)) or Hsp104Y662F (ΔRPR (SY3004), R1-5 (SY3002), RWT (SY3001), and R2E1 (SY3005)) were spotted on rich medium (1/4 YPD) and medium lacking adenine (SD-Ade) to analyze the [PSI+] phenotype. B. SDD-AGE was performed on lysates from the strains described in A, followed by immunoblotting for Sup35.
