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. 2016 Nov 4;12(11):e1006417. doi: 10.1371/journal.pgen.1006417

Fig 8. Sup35 repeats but not RPR exert transferrable effects on another Hsp104 substrate.

Fig 8

A. Schematic diagram of reporter construct. B. The indicated strains expressing luciferase reporter (RWT (SY2603), R1-4 (SY2666), R2E2 (SY2640), and ΔRPR (SY2637)) constructs were incubated at 37°C for 30 minutes, followed by 40°C for 35 minutes, with cycloheximide added for the last 10 minutes, in a microfluidics chamber. Cells were then allowed to recover at 30°C in the presence of cycloheximide, and images were taken at the indicated time points. At each time point, the percentage of cells containing GFP foci was determined.