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. 2016 Nov 4;12(11):e1005952. doi: 10.1371/journal.ppat.1005952

Fig 5. Minimal impact of bradykinin treatment on fungal clearance and inflammatory cells influx in the C. albicans infected kidney.

Fig 5

WT mice were treated ± bradykinin (300 nmol/kg/day) or PBS on day -1 (relative to infection). (A) Kidneys were evaluated for fungal load on days 3 (n = 3–4) and 7 (n = 8–11) p.i. (B) At day 7 p.i. (n = 4–6), kidney infiltrating neutrophils (Gr1+) and macrophages (F4/80+) (gated on CD45+) cells were evaluated by flow cytometry. Data are combined from 2 independent experiments for (A) and (B). Each dot represents one mouse, and the bars indicate mean. (C) BM-derived neutrophils and BMDMs from WT mice were incubated in vitro with unopsonized C. albicans yeast ± bradykinin for 3 h, and fungal load was determined by plating culture supernatants. Percentage of C. albicans killed by neutrophils and macrophages is shown. (D) IL-6 in RTEC conditioned media was assessed after 24 h bradykinin treatment. (E) IL-6 and TNFα and (F) nitrite levels in the supernatants of BMDMs treated ± bradykinin or LPS (1 ng/ml) for 24 h. Data are representative of 3 independent experiments for (C-F). Bars indicate mean ± S.D. p <0.01 (**). ns, not significant.