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. 2016 Nov 4;11(11):e0165965. doi: 10.1371/journal.pone.0165965

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© 2016 Bartholomäus et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — An unrooted phylogenetic tree of the Narnaviridae was calculated based on the alignment of the RdRp domain spanning the conserved motifs A to F using the neighbor-joining method with 1000 bootstrap replicates. The scale represents a genetic distance of 0.1 amino acid substitutions per site. Bootstrap values < 50% are not shown. Analyzed species include mitoviruses identified in Rhizoctonia as well as species of the family Narnaviridae recognized by the ICTV. Species identified in this study are marked with dots. Analysis was conducted using MEGA 6.06 and the sequence alignment algorithm MUSCLE [45]. CpMV-1, Cryphonectria parasitica mitovirus (NP_660174.1); OMV-3a, Ophiostoma mitovirus 3a (CAJ32468.1); OMV-4, Ophiostoma mitovirus 4 (NP_660179.1); OMV-5, Ophiostoma mitovirus 5 (NP_660180.1); OMV-6, Ophiostoma mitovirus 6 (NP_660181.1); ScNV-20S, Saccharomyces 20S RNA narnavirus (NP_660178.1); ScNV-23S, Saccharomyces 23S RNA narnavirus (NP_660177.1); RsMV-1, Rhizoctonia solani mitovirus 1 (ALD89121.1); TcMV-1, Thanatephorus cucumeris mitovirus 1 (AAD17381.1); RsMV-2, Rhizoctonia solani mitovirus 2 (ALD89121.1); RsMV-3, Rhizoctonia solani mitovirus 3 (ALD89122.1); RsMV-4, Rhizoctonia solani mitovirus 4 (ALD89123.1); RsMV-5, Rhizoctonia solani mitovirus 5 (ALD89124.1); RsMV-6, Rhizoctonia solani mitovirus 6 (ALD89125.1); RsMV-7, Rhizoctonia solani mitovirus 7 (ALD89126.1); RsMV-8, Rhizoctonia solani mitovirus 8 (ALD89127.1); RsMV-9, Rhizoctonia solani mitovirus 9 (ALD89128.1); RsMV-10, Rhizoctonia solani mitovirus 10 (ALD89102.1); RsMV-11, Rhizoctonia solani mitovirus 11 (ALD89116.1); RsMV-12, Rhizoctonia solani mitovirus 12 (ALD89117.1); RsMV-13, Rhizoctonia solani mitovirus 13 (ALD89118.1); RsMV-14, Rhizoctonia solani mitovirus 14 (ALD89119.1); RsMV-15, Rhizoctonia solani mitovirus 15 (ALD89120.1); RsMV-K1, Rhizoctonia solani mitovirus K1 (ALD60243.1); RcMV, Rhizoctonia cerealis mitovirus (AIT71973.1); RsMV-9, Rhizoctonia solani mitovirus 9 DC17 (KX349058); RsMV-16, Rhizoctonia solani mitovirus 16 (KX349057); RsMV-17, Rhizoctonia solani mitovirus 17 (KX349059); RsMV-18, Rhizoctonia solani mitovirus 18 (KX349060); RsMV-19, Rhizoctonia solani mitovirus 19 (KX349056); RsMV-20, Rhizoctonia solani mitovirus 20 (KX349062).