The RdRp domains of Rhizoctonia solani flexivirus 1 (RsFV-1) (KX349064), Rhizoctonia solani flexivirus 2 (RSFV-2) (KX349069) and the type species of all genera within the Tymovirales [8] were aligned using MEGA 6.06 and the sequence alignment algorithm MUSCLE [44]. Conserved motifs A to G were marked according to Bruenn 2003, Černý et al. 2014, Koonin et al. 1993, Boehr et al. 2014 and Xu et al. 2003 [30, 34, 42–44]. ACLSV, Apple chlorotic leaf spot virus (ABY71563.1); AcLV, Aconitum latent virus (NP_116487.1); ASGV, Apple stem grooving virus (BAA98054.1); ASPV, Apple stem pitting virus (AEP02955.1); BotV-F, Botrytis virus F (NP_068549.1); BotV-X, Botrytis virus X (NP_932306.1); CLBV, Citrus leaf blotch virus (NP_624333.1); GFkV, Grapevine fleck virus (NP_542612.1); GVA, Grapevine virus A (AAO17778.1); ICRSV, Indian citrus ringspot virus (NP_203553.1); LoLV, Lolium latent virus (YP_001718499.1); MRFV, Maize rayado fino virus (NP_115454.1); PVT, Potato virus T (BAM16482.1); PVX, Potato virus X (AAF89747.1); SsDRV, Sclerotinia sclerotiorum debilitation-associated RNA virus (YP_325662.1); ShVX, Shallot virus X (NP_620648.1). Shading indicates level of conservation and the consensus sequence is displayed.