Figure 1.

The absorbance and emission spectra of AM ether fluorogenic probes. The absorbance curves are shown as solid lines, with emission curves shown as dashed lines. Each sample was prepared as 1 μM probe in 10 mM HEPES (pH 7.3). A) Spectra for DDAO (λ_ex = 600 nm), DDAO-AME 1 (λ_ex = 465 nm), and DDAO-AME 2 (nonfluorescent). B) Spectra for resorufin (λ_ex = 525 nm) and Res-AME (λ_ex = 475 nm).