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Kinetic parameters of DDAO-AME 1 and Res-AME with esterases and lipases.
| Probe | Enzyme (amount [μg mL−1]) | Vmax [pmol s−1] | KM [μM] |
|---|---|---|---|
| DDAO-AME 1 | PLE (0.5) | 1.2 | 7.5 ± 0.8 |
| B. subtilis esterase (0.5) | 0.45 | 6.7 ± 0.5 | |
| C. antarctica lipase (10) | 2.4 | 9.5 ± 1.1 | |
| M. miehei lipase (10) | 0.06 | 4.4 ± 0.7 | |
| Res-AME | PLE (0.050) | 0.69 | 4.9 ± 0.7 |
| B. subtilis esterase (0.5) | 0.30 | 19 ± 2 | |
| C. antarctica lipase (10) | 0.30 | 6.8 ± 0.4 | |
| M. miehei lipase (20) | 0.16 | 19 ± 3 |
