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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2016 Apr 16;42:107–118. doi: 10.1016/j.dnarep.2016.04.002

Figure 3. LoVo extracts have reduced SV40 replication efficiency, and yield more slow-migrating replication products.

Figure 3

A) The efficiency of replication by LoVo extracts was considerably reduced compared to the MMR-proficient HeLa cell extracts: Compare the amounts of fully-replicated products (linearized, see arrow) to the unreplicated or partially replicated material digested by DpnI (DpnI cleaves bi-methylated adenosine residues at GATC sites of bacterial but not primate replication). B) Replication products produced in the presence of all four radionucleotides were assessed for their electrophoretic migration following release of the repeat-containing fragment and resolved on 4% PAGE. Slipped-DNA heteroduplexes migrate slower than fully-duplexed species [76]. A slow-migrating smear representing a high-proportion of the repeat-containing fragment was evident for the LoVo replicated material compared to the HeLa replicated material. Moreover, a distinct species was just evident in the LoVo but not HeLa replicated material, see arrow.