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. Author manuscript; available in PMC: 2016 Nov 4.
Published in final edited form as: Chembiochem. 2016 Jul 13;17(17):1636–1642. doi: 10.1002/cbic.201600265

Figure 3.

Figure 3

Impact of oxygen-to-sulfur substitution at Lys57 interaction site on binding affinity for the Antp homeodomain-DNA complex. (A) Competition assay data used to determine Kd constants for RP and SP-monothioated DNA duplexes. The total concentrations of the probe DNA and the protein were 10 and 50 nM, respectively in this experiment. The measurement was repeated four times. The solid lines represent best-fit curves. (B) Change in the binding free energy upon modifications of the DNA phosphate group at the Lys57 interaction site. In this case, ΔΔG is defined as ΔG (unmodified) – ΔG (modified). Data for the dithioated DNA duplexes are from our previous study.[5d]