Figure 1. NLRC4 protects against B16F10 tumor growth in vivo.

(A–F) WT and Nlrp6^–/– (A), Nlrp12^–/– (B), Nlrp3^–/– (C), and Nlrc4^–/– (D–F) mice were injected s.c. with 1 × 10⁵ B16F10 cells. (A–C and E) Tumor mass was determined at 16 to 20 days after inoculation. (D) WT and Nlrc4^–/– tumor areas (length × width) were measured every 2 to 3 days. (F) Representative images of excised WT and Nlrc4^–/– B16F10 tumors. (A–E) Data are representative of 3 independent experiments with n = 5 mice per group. (A–C and E) *P ≤ 0.05, unpaired 2-tailed Student’s t test. (D) Error bars represent SEM. **P ≤ 0.01, ****P ≤ 0.0001, 2-way ANOVA with Šidák’s multiple comparisons test.